Immunological Properties of Rickettsia rickettsii Purified by Zonal Centrifugation
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چکیده
منابع مشابه
Immunological properties of Rickettsia rickettsii purified by zonal centrifugation.
The properties of Rickettsia rickettsii purified from infected chicken yolk sacs or mouse L cell cultures by sucrose density gradient centrifugation in a zonal rotor were examined in various ways. Rickettsiae derived from both L cells and yolk sacs had similar compositions: about 12% nitrogen, 1.5% phosphorus, 5% carbohydrate, and 30% fatty acids. On a dry-weight basis, purified rickettsiae wer...
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The ability of the spotted fever agent, Rickettsia rickettsii, to grow not only in the cytoplasm but also in the nucleus of infected cells was first noted by Wolbach (12) in epithelial cells of the gut, hypoderm and salivary gland of infected ticks; it has subsequently been established for all spotted fever group rickettsiae investigated, especially when they were maintained on mammalian tissue...
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In this paper, we describe a cultivation procedure in large-capacity fermentors (200 liters) and the purification of the Salmonella crude extracts by means of zonal centrifugation in a sucrose gradient. Briefly, the endotoxin has been extracted from salmonellae with hypertonic solutions. The crude endotoxin was shown to contain several antigens, mainly r(1), r(2), r(3), or heterologous antigen....
متن کاملCharacterization of the Madrid E strain of Rickettsia prowazekii purified by renografin density gradient centrifugation.
The avirulent Madrid E strain of Rickettsia prowazekii cultivated in chicken yolk sacs could be purified successfully with a Renografin density gradient method developed previously for Rickettsia typhi. Recovery during purification, viability, and lack of contamination with host cell components were similar for the two species, although yields of R. prowazekii per yolk sac were lower. Purified ...
متن کاملPlaque assay for Rickettsia rickettsii.
A plaque technique for the assay of Rickettsia rickettsii is described. The method employs primary chick or green monkey kidney monolayer cell cultures with either an agarose or special Noble agar overlay. Plaques were counted in 6 days and resultant titers correlated well with ld(50) end points obtained by a standard assay in embryonated eggs. Identification of the plaque-forming organisms was...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1975
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.11.6.1203-1209.1975